Frontispiece: Highly Multiplexed Single-Cell Protein Analysis
نویسندگان
چکیده
منابع مشابه
Highly multiplexed single-cell quantitative PCR
We present a microfluidic device for rapid gene expression profiling in single cells using multiplexed quantitative polymerase chain reaction (qPCR). This device integrates all processing steps, including cell isolation and lysis, complementary DNA synthesis, pre-amplification, sample splitting, and measurement in twenty separate qPCR reactions. Each of these steps is performed in parallel on u...
متن کاملHighly multiplexed single-cell analysis of formalin-fixed, paraffin-embedded cancer tissue.
Limitations on the number of unique protein and DNA molecules that can be characterized microscopically in a single tissue specimen impede advances in understanding the biological basis of health and disease. Here we present a multiplexed fluorescence microscopy method (MxIF) for quantitative, single-cell, and subcellular characterization of multiple analytes in formalin-fixed paraffin-embedded...
متن کاملMultiplexed single-cell in situ RNA analysis by reiterative hybridization
Most current approaches for quantification of RNA species in their natural spatial contexts in single cells are limited by a small number of parallel analyses. Here we report a strategy to dramatically increase the multiplexing capacity for RNA analysis in single cells in situ. In this method, transcripts are detected by fluorescence in situ hybridization (FISH). After imaging and data storage,...
متن کاملجداسازی مخمرهای تجزیه کننده آلکانها و بررسی خواص سیتوکروم و پروتئین تک آنها ( single cell protein) یاخته
نفت خام با بیش از340 فراورده یکی از اصلی ترین منابع انرژی و نیروی محرکه اقتصادی جهانی است و ایران 9 درصد از کل مخازن نفت جهان را در اختیار دارد. با توجه به فراوانی دفعاتی که یک تانکر یا بشکه ی نفت منتقل و ذخیره می شود، تصادف و نشت آن اجتناب ناپذیر است. احتمالاً کل میزان نفتی که از طریق فعالیّت های انسانی و یا طبیعی وارد دریا می شود می تواند سطح همه ی اقیانوس ها تا ضخامت20 مولکول بپوشاند. پاکسازی ...
Highly multiplexed and quantitative cell-surface protein profiling using genetically barcoded antibodies
Human cells express thousands of different surface proteins that can be used for cell classification, or to distinguish healthy and disease conditions. A method capable of profiling a substantial fraction of the surface proteome simultaneously and inexpensively would enable more accurate and complete classification of cell states. We present a highly multiplexed and quantitative surface proteom...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Chemistry - A European Journal
سال: 2018
ISSN: 0947-6539
DOI: 10.1002/chem.201882862